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Nacalai stripping solution strong
Stripping Solution Strong, supplied by Nacalai, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/stripping+solution+strong/solution+stripping/pmc13194054-88-16-19
Average 86 stars, based on 1 article reviews
stripping solution strong - by Bioz Stars, 2026-11
86/100 stars

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Related Articles

Control:

Article Title: Plasmodium falciparum diacylglycerol acyltransferase maintains phospholipid homeostasis to regulate sexual differentiation, ER stress, and cytoadhesion
Article Snippet: Signals were detected using chemiluminescent HRP substrate (Thermo Fisher Scientific) and visualized with WSE-6100 LuminoGraph I (ATTO, Tokyo, Japan). .. For internal control detection of PfAldolase, membranes were stripped with WB Stripping Solution Strong (Nacalai Tesque) for 30 minutes, re-blocked with Bullet Blocking One, incubated with HRP-conjugated anti- Plasmodium aldolase antibody for 30 minutes, and the signal was developed with chemiluminescent HRP substrate (Thermo Fisher Scientific) for 5 minutes. ..

Article Title: Deciduous pulp stem cell-derived extracellular vesicles stimulate the proliferation of cartilage progenitor cells via extracellular signal-regulated protein kinase 1/2 activation.
Article Snippet: The cells were then incubated with an HRPconjugated secondary antibody (1:1000; Santa Cruz Biotechnology, Santa Cruz, CA, USA) for 1 h at room temperature. .. The membranes were treated with WB Stripping Solution Strong (Nacalai Tesque) and stained with an antiactin beta antibody (Merck) as an internal control, followed by incubation with HRP-conjugated secondary antibody (1:1000; Santa Cruz Biotechnology). .. The membrane images were acquired using SuperSignal West Pico (Thermo Fisher Scientific) on a membrane imager, ImageQuant LAS 4010 (GE Healthcare Life Sciences, Pittsburgh, PA, USA) or ImageQuant 800 (Cytiba, Marlborough, MA, USA).

Western Blot:

Article Title: Plasmodium falciparum diacylglycerol acyltransferase maintains phospholipid homeostasis to regulate sexual differentiation, ER stress, and cytoadhesion
Article Snippet: Signals were detected using chemiluminescent HRP substrate (Thermo Fisher Scientific) and visualized with WSE-6100 LuminoGraph I (ATTO, Tokyo, Japan). .. For internal control detection of PfAldolase, membranes were stripped with WB Stripping Solution Strong (Nacalai Tesque) for 30 minutes, re-blocked with Bullet Blocking One, incubated with HRP-conjugated anti- Plasmodium aldolase antibody for 30 minutes, and the signal was developed with chemiluminescent HRP substrate (Thermo Fisher Scientific) for 5 minutes. ..

Article Title: Deciduous pulp stem cell-derived extracellular vesicles stimulate the proliferation of cartilage progenitor cells via extracellular signal-regulated protein kinase 1/2 activation.
Article Snippet: The cells were then incubated with an HRPconjugated secondary antibody (1:1000; Santa Cruz Biotechnology, Santa Cruz, CA, USA) for 1 h at room temperature. .. The membranes were treated with WB Stripping Solution Strong (Nacalai Tesque) and stained with an antiactin beta antibody (Merck) as an internal control, followed by incubation with HRP-conjugated secondary antibody (1:1000; Santa Cruz Biotechnology). .. The membrane images were acquired using SuperSignal West Pico (Thermo Fisher Scientific) on a membrane imager, ImageQuant LAS 4010 (GE Healthcare Life Sciences, Pittsburgh, PA, USA) or ImageQuant 800 (Cytiba, Marlborough, MA, USA).

Article Title: Phosphoproteomics identifies Rho-kinase-associated phospho-signaling and autophagy-associated alterations in the medial prefrontal cortex of a schizophrenia-related mouse model.
Article Snippet: .. For sequential detection of phosphorylated and total protein, membranes were stripped using WB Stripping Solution Strong (Cat# 05677–65; Nacalai Tesque) at room temperature for 30 min and then reprobed under the same conditions. ..

Article Title: Regulatory mechanisms of mitochondrial function by cancer-derived exosomes in cachexia
Article Snippet: Protein signals were visualized using ECL reagents (Cytiva, Marlborough, MA, USA), captured with the ImageQuant LAS 500 system (Cytiva), and analyzed with Image Studio Digits v5.2 (LI-COR). .. When necessary, after detection of the target proteins, the same PVDF membranes were treated with WB Stripping Solution Strong (Nacalai Tesque) for 15 min at room temperature to remove bound antibodies. ..

Article Title: Protocol for differentiating patient-derived iPSCs into photoreceptor-like cells
Article Snippet: SuperSignal West Femto Maximum Sensitivity Substrate , Thermo Fisher Scientific , Cat# 34095. .. WB Stripping Solution Strong , Nacalai tesque , Cat# 05677-65. .. Mini-PROTEAN TGX Any kD , Bio-Rad , Cat# 4569036.

Article Title: Kinetochore Homeostasis is Maintained by Coordinated Chromatin Stabilization and Soluble Buffering
Article Snippet: Image processing and quantification were performed using Image Lab v5.2.1 (Bio-Rad). .. For membrane stripping and reprobing, membranes were rinsed in 0.1% TBST for 10 min, then incubated in WB Stripping Solution Strong (Nacalai Tesque), pre-heated to 55 °C, for 20-40 min with gentle agitation. ..

Stripping Membranes:

Article Title: Plasmodium falciparum diacylglycerol acyltransferase maintains phospholipid homeostasis to regulate sexual differentiation, ER stress, and cytoadhesion
Article Snippet: Signals were detected using chemiluminescent HRP substrate (Thermo Fisher Scientific) and visualized with WSE-6100 LuminoGraph I (ATTO, Tokyo, Japan). .. For internal control detection of PfAldolase, membranes were stripped with WB Stripping Solution Strong (Nacalai Tesque) for 30 minutes, re-blocked with Bullet Blocking One, incubated with HRP-conjugated anti- Plasmodium aldolase antibody for 30 minutes, and the signal was developed with chemiluminescent HRP substrate (Thermo Fisher Scientific) for 5 minutes. ..

Article Title: Deciduous pulp stem cell-derived extracellular vesicles stimulate the proliferation of cartilage progenitor cells via extracellular signal-regulated protein kinase 1/2 activation.
Article Snippet: The cells were then incubated with an HRPconjugated secondary antibody (1:1000; Santa Cruz Biotechnology, Santa Cruz, CA, USA) for 1 h at room temperature. .. The membranes were treated with WB Stripping Solution Strong (Nacalai Tesque) and stained with an antiactin beta antibody (Merck) as an internal control, followed by incubation with HRP-conjugated secondary antibody (1:1000; Santa Cruz Biotechnology). .. The membrane images were acquired using SuperSignal West Pico (Thermo Fisher Scientific) on a membrane imager, ImageQuant LAS 4010 (GE Healthcare Life Sciences, Pittsburgh, PA, USA) or ImageQuant 800 (Cytiba, Marlborough, MA, USA).

Article Title: Phosphoproteomics identifies Rho-kinase-associated phospho-signaling and autophagy-associated alterations in the medial prefrontal cortex of a schizophrenia-related mouse model.
Article Snippet: .. For sequential detection of phosphorylated and total protein, membranes were stripped using WB Stripping Solution Strong (Cat# 05677–65; Nacalai Tesque) at room temperature for 30 min and then reprobed under the same conditions. ..

Article Title: Regulatory mechanisms of mitochondrial function by cancer-derived exosomes in cachexia
Article Snippet: Protein signals were visualized using ECL reagents (Cytiva, Marlborough, MA, USA), captured with the ImageQuant LAS 500 system (Cytiva), and analyzed with Image Studio Digits v5.2 (LI-COR). .. When necessary, after detection of the target proteins, the same PVDF membranes were treated with WB Stripping Solution Strong (Nacalai Tesque) for 15 min at room temperature to remove bound antibodies. ..

Article Title: Kinetochore Homeostasis is Maintained by Coordinated Chromatin Stabilization and Soluble Buffering
Article Snippet: Image processing and quantification were performed using Image Lab v5.2.1 (Bio-Rad). .. For membrane stripping and reprobing, membranes were rinsed in 0.1% TBST for 10 min, then incubated in WB Stripping Solution Strong (Nacalai Tesque), pre-heated to 55 °C, for 20-40 min with gentle agitation. ..

Blocking Assay:

Article Title: Plasmodium falciparum diacylglycerol acyltransferase maintains phospholipid homeostasis to regulate sexual differentiation, ER stress, and cytoadhesion
Article Snippet: Signals were detected using chemiluminescent HRP substrate (Thermo Fisher Scientific) and visualized with WSE-6100 LuminoGraph I (ATTO, Tokyo, Japan). .. For internal control detection of PfAldolase, membranes were stripped with WB Stripping Solution Strong (Nacalai Tesque) for 30 minutes, re-blocked with Bullet Blocking One, incubated with HRP-conjugated anti- Plasmodium aldolase antibody for 30 minutes, and the signal was developed with chemiluminescent HRP substrate (Thermo Fisher Scientific) for 5 minutes. ..

Incubation:

Article Title: Plasmodium falciparum diacylglycerol acyltransferase maintains phospholipid homeostasis to regulate sexual differentiation, ER stress, and cytoadhesion
Article Snippet: Signals were detected using chemiluminescent HRP substrate (Thermo Fisher Scientific) and visualized with WSE-6100 LuminoGraph I (ATTO, Tokyo, Japan). .. For internal control detection of PfAldolase, membranes were stripped with WB Stripping Solution Strong (Nacalai Tesque) for 30 minutes, re-blocked with Bullet Blocking One, incubated with HRP-conjugated anti- Plasmodium aldolase antibody for 30 minutes, and the signal was developed with chemiluminescent HRP substrate (Thermo Fisher Scientific) for 5 minutes. ..

Article Title: Deciduous pulp stem cell-derived extracellular vesicles stimulate the proliferation of cartilage progenitor cells via extracellular signal-regulated protein kinase 1/2 activation.
Article Snippet: The cells were then incubated with an HRPconjugated secondary antibody (1:1000; Santa Cruz Biotechnology, Santa Cruz, CA, USA) for 1 h at room temperature. .. The membranes were treated with WB Stripping Solution Strong (Nacalai Tesque) and stained with an antiactin beta antibody (Merck) as an internal control, followed by incubation with HRP-conjugated secondary antibody (1:1000; Santa Cruz Biotechnology). .. The membrane images were acquired using SuperSignal West Pico (Thermo Fisher Scientific) on a membrane imager, ImageQuant LAS 4010 (GE Healthcare Life Sciences, Pittsburgh, PA, USA) or ImageQuant 800 (Cytiba, Marlborough, MA, USA).

Article Title: Kinetochore Homeostasis is Maintained by Coordinated Chromatin Stabilization and Soluble Buffering
Article Snippet: Image processing and quantification were performed using Image Lab v5.2.1 (Bio-Rad). .. For membrane stripping and reprobing, membranes were rinsed in 0.1% TBST for 10 min, then incubated in WB Stripping Solution Strong (Nacalai Tesque), pre-heated to 55 °C, for 20-40 min with gentle agitation. ..

Staining:

Article Title: Deciduous pulp stem cell-derived extracellular vesicles stimulate the proliferation of cartilage progenitor cells via extracellular signal-regulated protein kinase 1/2 activation.
Article Snippet: The cells were then incubated with an HRPconjugated secondary antibody (1:1000; Santa Cruz Biotechnology, Santa Cruz, CA, USA) for 1 h at room temperature. .. The membranes were treated with WB Stripping Solution Strong (Nacalai Tesque) and stained with an antiactin beta antibody (Merck) as an internal control, followed by incubation with HRP-conjugated secondary antibody (1:1000; Santa Cruz Biotechnology). .. The membrane images were acquired using SuperSignal West Pico (Thermo Fisher Scientific) on a membrane imager, ImageQuant LAS 4010 (GE Healthcare Life Sciences, Pittsburgh, PA, USA) or ImageQuant 800 (Cytiba, Marlborough, MA, USA).

Stripping:

Article Title: Protocol for differentiating patient-derived iPSCs into photoreceptor-like cells
Article Snippet: SuperSignal West Femto Maximum Sensitivity Substrate , Thermo Fisher Scientific , Cat# 34095. .. WB Stripping Solution Strong , Nacalai tesque , Cat# 05677-65. .. Mini-PROTEAN TGX Any kD , Bio-Rad , Cat# 4569036.

Membrane:

Article Title: Kinetochore Homeostasis is Maintained by Coordinated Chromatin Stabilization and Soluble Buffering
Article Snippet: Image processing and quantification were performed using Image Lab v5.2.1 (Bio-Rad). .. For membrane stripping and reprobing, membranes were rinsed in 0.1% TBST for 10 min, then incubated in WB Stripping Solution Strong (Nacalai Tesque), pre-heated to 55 °C, for 20-40 min with gentle agitation. ..

Gentle:

Article Title: Kinetochore Homeostasis is Maintained by Coordinated Chromatin Stabilization and Soluble Buffering
Article Snippet: Image processing and quantification were performed using Image Lab v5.2.1 (Bio-Rad). .. For membrane stripping and reprobing, membranes were rinsed in 0.1% TBST for 10 min, then incubated in WB Stripping Solution Strong (Nacalai Tesque), pre-heated to 55 °C, for 20-40 min with gentle agitation. ..



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